Pfu DNA Polymerase

1.Pfu DNA Polymerase is one of the most commonly used high-fidelity DNA polymerases.
2.Pfu DNA Polymerase is a highly heat-stable DNA polymerase derived from Pyrococcus furiosus. The molecular weight of Pfu enzyme is 90kDa. Pfu enzymes can catalyze the polymerization of deoxynucleotides in the 5' to 3' direction, depending on the DNA template. Unlike Taq enzymes, Pfu enzymes have a 3' to 5' proofreading activity. In addition, the Pfu enzyme has 5' to 3' exonuclease activity, but the Pfu enzyme has no reverse transcriptase activity.
3.Because the Pfu enzyme has a 3' to 5' exonuclease activity, the chance of error during PCR amplification is greatly reduced, with an error rate of 2.6×10-6 per nt per cycle. The error rate of Pfu is not only much lower than Taq enzyme, but also lower than some other high-fidelity DNA polymerases such as Vent, DeepVent, Pwo, Tli, etc. Therefore, Pfu enzyme is often used as the preferred cost-effective high-fidelity DNA polymerase.

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